For isolation and enumeration of Enterobacteriaceae and Bacillus cereus.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Greenish beige color
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Purple colored, slightly opalescent gel
pH
7.0 ± 0.2 at 25˚C (41.52 g/ l)
Solidification
40˚C
Description
A fine, free flowing, hygroscopic powder
Solubility
Soluble in Distilled/de-ionized water at 50°C
Solidification
40 ˚C
Contents
Ingredients
Gram/Litre
Casein enzymic hydrolysate
10.000
Yeast extract
1.500
Glucose
10.000
Sodium chloride
5.000
Bromocresol purple
0.015
Agar
15.00
pH at 25˚C
7.0±0.2
Directions
Dissolve 41.52 grams in 1000 ml distilled water. Boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in petri plates or tube for slant preparation. Ensure complete solidification and inoculate test sample aseptically.
