Light green opalescent with white precipitate, on standing the precipitate settles down
pH
7.0 ± 0.2 at 25˚C (63.07 g/ l)
Contents
Ingredients
Gram/Litre
Peptone
8.600
Sodium chloride
6.400
Ox Bile
8.000
Calcium Carbonate
20.000
Potassium tetrathionate
20.000
Brilliant green
0.070
pH at 25˚C
7.0 ± 0.2
Directions
Dissolve 63.07 gram in 1000 ml distilled water. Heat just too boiling. Do Not
Autoclave or Overheat. Cool below 45-50˚C, and distribute aseptically in sterile test tubes or as
desired and inoculate test sample aseptically.