For isolation of Salmonellae from faecal samples, sewage and other samples.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Green colored opalescent solution
Color (Complete medium with added iodine solution)
Light green opalescent with white precipitate, on standing the precipitate settles down. (77.50 g i
Contents
Ingredients
Gram/Litre
Meat Extract
0.900
Peptone
4.500
Yeast Extract
1.800
Sodium Chloride
4.500
Calcium Carbonate
25.000
Sodium Thiosulphate
40.700
Brilliant green
0.012
Directions
Dissolve 77.50 g in 980 ml distilled water. Heat just too boiling. Do Not Autoclave or
Overheat. Cool below 45-50 ˚C, add 20 ml iodine solution, mix well and distribute aseptically in
sterile test tubes and inoculate test sample aseptically.
Preparation of Iodine solution: dissolve 5 g of potassium iodide and 6 g of iodine in 20 ml distilled
water).
