For the selective isolation of Gonococci from clinical samples.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Basal medium: Light amber, slightly opalescent gel
pH
7.0 ±0.2 at 25 ˚C (42.00 g/ l)
Solidification
40˚C
Color
Basal medium: Light amber, slightly opalescent gel. With lysed blood or 2% hemoglobin: cherry red, o
Contents
Ingredients
Gram/Litre
Special peptone
23.000
Starch
1.000
Sodium Chloride
5.000
Agar
13.000
pH at 25˚C
7.0 ±0.2
Directions
Dissolve 42.00 grams in 900 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C,
and aseptically add 100 ml sterilized lysed blood and two vials of Vitamino growth supplement
and V.C.N. supplement. Mix well and pour into sterile petri plates. Ensure complete
solidification and inoculate test sample aseptically.
Or
If Hemoglobin is used, dissolve 42.0 grams of medium in in 500 ml distilled water. Heat to boiling
to dissolve the medium completely. Prepare 500 ml of 2% hemoglobin solution and sterilize
separately by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix both
and add two vials of Vitamino growth supplement and V.C.N. supplement
For selectivity the media is fortified with B or VX supplement, CNV antimicrobial solution or
CNVT antimicrobial solution.
