For selective isolation and differentiation of Corynebacterium diphtheriae
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Light amber colored clear to slightly opalescent solution
pH
7.40 ±0.2 at 25 ˚C (40.50 g/ l)
Solidification
40˚C
Color
Light amber colored clear to slightly opalescent gel.
Contents
Ingredients
Gram/Litre
Peptic digest of animal tissue
20.00
Sodium chloride
5.00
L-cystine
0.24
Sodium thiosulphate
0.43
Agar
15.00
pH at 25˚C
7.4 ±0.2
Directions
Dissolve 40.50 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45°C
and add heat sensitive compounds. Aseptically add sterile horse serum (100 ml) 1 ml Potassium
Tellurite, mix well and pour into sterile Petri plates.
