Todd Hewitt Broth

ARTICLE NO. OLFC-1833
HSN NO. 38210000


Pack Size

Size
100 GM
500 GM

Application

For cultivation of group A haemolytic Streptococci used for serological studies.


Specifications

PHYSICAL PARAMETERS
Description A fine, free flowing, hygroscopic powder.
Color Light beige colored powder.
Solubility Soluble in Distilled / de-ionized water at 50˚ C
Color Light to medium amber colored, clear solution.
pH 7.8 ± 0.2 at 25℃ (37.00 g/ l)

Contents

Ingredients Gram/Litre
Beef Heart, Infusion form500.000
Peptone20.000
Dextrose2.000
Sodium Chloride2.000
Disodium hydrogen phosphate0.400
Sodium carbonate2.500
pH at 25˚C7.8 ± 0.2


Directions

Dissolve 37.0 grams in 1000 ml distilled water. Boil to dissolve the medium completely and distribute as desired. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.