For cultivation of group A haemolytic Streptococci used for serological studies.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Light to medium amber colored, clear solution.
pH
7.8 ± 0.2 at 25℃ (37.00 g/ l)
Contents
Ingredients
Gram/Litre
Beef Heart, Infusion form
500.000
Peptone
20.000
Dextrose
2.000
Sodium Chloride
2.000
Disodium hydrogen phosphate
0.400
Sodium carbonate
2.500
pH at 25˚C
7.8 ± 0.2
Directions
Dissolve 37.0 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and distribute as desired. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15
min, cool it to 42-45 °C and inoculate test sample aseptically.