For a cultivation and maintenance of Salmonella typhi.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Light amber, opalescent solution
pH
7.3 ±0.2 at 25℃ (45.00 g/ l)
Solidification
40˚C
Color
Light amber, clear opalescent gel
Contents
Ingredients
Gram/Litre
Tryptone
17.00
Soya Peptone
3.00
Sodium Chloride
5.00
Dextrose
2.50
Dipotassium hydrogen phosphate
2.50
Agar
15.00
pH at 25˚C
7.3 ±0.2
Directions
Dissolve 45.00 grams in 1000 ml distilled water. Boil to dissolve the medium
completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45°C and
distribute aseptically in petri plates and allow to solidify. Ensure complete solidification and
inoculate test sample aseptically.
