For rapid detection and enumeration of Escherichia coli in foods using direct plating
method.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Light amber colored opalescent
pH
7.2 ±0.2 at 25˚C (36.50 g/ l)
Solidification
40˚C
Color
Light amber colored opalescent gel
Contents
Ingredients
Gram/Litre
Tryptone
20.00
Bile salt
1.50
Agar
15.00
pH at 25˚C
7.2 ±0.2
Directions
Dissolve 36.50 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C
and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample
aseptically.
