A selective and differential medium used in sanitary bacteriology to enrichment
Salmonella & Shigella from feces, urine, and fresh and canned foods.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light buff to pink colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Red-orange, clear solution
pH
7.0 ±0.2 at 25˚C (46.56 g/ l)
Contents
Ingredients
Gram/Litre
Proteose peptone
5.000
Meat extract
5.000
Lactose
10.000
Bile salts
8.500
Sodium citrate
8.500
Sodium thio-sulphate
8.500
Ferric citrate
1.000
Neutral red
0.025
Brilliant green
0.033
pH at 25˚C
7.0 ±0.2
Directions
Dissolve 46.56 grams in 1000 ml distilled water. Boil to dissolve the medium
completely for sterilization. DO NOT AUTOCLAVE, cool it to 42-45 °C and distribute
aseptically in to the sterile test tubr and inoculate aseptically.
