A modified medium, which provides better growth of Salmonella and Shigella species.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light buff to pink colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Red-orange, slightly opalescent solution
pH
7.2 ±0.2 at 25 ˚C (57.00 g/ l)
Solidification
40˚C
Color
Red-orange, slightly opalescent gel
Contents
Ingredients
Gram/Litre
Proteose peptone
5.000
Meat extract
5.000
Lactose
10.000
Bile salts mixture
5.500
Sodium citrate
10.000
Sodium thio-sulphate
8.500
Ferric citrate
1.000
Neutral red
0.025
Brilliant green
0.033
Agar
12.000
pH at 25˚C
7.2 ±0.2
Directions
Dissolve 57.00 grams in 1000 ml distilled water. Boil to dissolve the medium
completely for sterilization. DO NOT AUTOCLAVE, cool it to 42-45 °C and distribute
aseptically in to the plates. Ensure complete solidification and inoculate aseptically.