For the isolation and cultivation of Shigella species from food stuff.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Light amber colored clear solution
pH
7.0 ±0.2 at 25 ˚C (31.50 g/ l)
Contents
Ingredients
Gram/Litre
Tryptose
20.000
Sodium chloride
5.000
Dipotassium phosphate
2.000
Potassium phosphate
2.000
Dextrose
1.000
Polysorbate 80
1.500
pH at 25˚C
7.0 ±0.2
Directions
Dissolve 31.50 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs pressure and 121 °C for 15 minutes, cool it to 42
45 °C add Novobiocin (0.550 mg), mix well and distribute aseptically in sterile test tubes and
inoculate test sample aseptically.
