For cultivation and studying haemolytic reactions of Bacillus cereus.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Dark amber, slightly opalescent
pH
7.3 ± 0.2 at 25 ˚C (37.5 g/ l)
Solidification
40˚C
Color
Without blood: medium to dark amber, slightly opalescent gel. With 5% blood: cherry red, opaque gel.
Contents
Ingredients
Gram/Litre
Soya peptone
5.000
Tryptone
15.000
Sodium Chloride
5.000
Agar
12.500
pH at 25˚C
7.3 ± 0.2
Directions
Dissolve 37.50 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs. pressure (121 °C) for 15 min, cool it to 42-45 °C,
and aseptically add 5% v/v sterile defibrinated blood. Mix well and pour into sterile petri plates.
Ensure complete solidification and inoculate test sample aseptically.
