For the heterotrophic plate count of treated potable water using longer incubation
periods.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Light amber colored opalescent
pH
7.2 ±0.2 at 25 ˚C (18.20 g/ l)
Solidification
40˚C
Color
Light amber colored opalescent gel
Contents
Ingredients
Gram/Litre
Yeast Extract
0.50
Proteose Peptone
0.50
Casamino Acids
0.50
Dextrose
0.50
Starch
0.50
Sodium Pyruvate
0.30
Dipotassium Phosphate
0.30
Magnesium Sulfate
0.05
Agar
15.00
pH at 25˚C
7.2 ±0.2
Directions
Dissolve 18.20 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45°C
and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample
aseptically.
