For isolation, cultivation and enumeration of Clostridia species, highly nutritive for
Clostridium sporogenes and other anaerobes.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Light amber colored opalescent medium
pH
6.8 ± 0.2 at 25℃ (38.00 g/ l)
Contents
Ingredients
Gram/Litre
Meat Extract
10.00
Peptone
10.00
Yeast Extract
3.00
Soluble Starch
1.00
Glucose Monohydrate
5.00
Cysteine Hydrocholoride
0.50
Sodium Chloride
5.00
Sodium Acetate
3.00
Agar
0.50
pH at 25˚C
6.8 ±0.2
Directions
Dissolve 38.00 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C
and distribute aseptically in petri plates and allow to solidify. Ensure complete solidification and
inoculate test sample aseptically.
