For selective isolation & enumeration of yeasts & molds from environmental materials &
foods.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Pinkish beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Pink colored opalescent
pH
7.2 ±0.2 at 25˚C (32.00 g/ l)
Solidification
40˚C
Color
Pink colored opalescent gel
Contents
Ingredients
Gram/Litre
Soya Peptone
5.00
Dextrose
10.00
Monopotassium Phosphate
1.00
Magnesium Sulfate
0.50
Rose Bengal
0.05
Agar
15.00
pH at 25˚C
7.2 ±0.2
Directions
Dissolve 32.00 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C
and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample
aseptically.
Note: For more selectivity add 2 ml of chloramphenicol solution (0.1 g in 2ml of ethanol) in
cooled agar base mix thoroughly and dispense in sterile petri plates.
