For the isolation and cultivation of Mycobacterium species.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Blue to greenish blue colored powder.
Solubility
The mixture of sterile basal medium and whole egg emulsion, yield pale bluish green colored
Color
Light yellowish to creamy colored opalescent
Solidification
40˚C
Color
The mixture of sterile basal medium and whole egg emulsion, coagulates to yield pale bluish green co
Contents
Ingredients
Gram/Litre
L-Asparagine
3.60
Monopotassium Phosphate
2.40
Magnesium Sulphate
0.24
Magnesium Citrate
0.60
Potato Starch Soluble
30.00
Malachite Green
0.40
Directions
Dissolve 37.3 gm of the medium in 600 ml of distilled water containing 12 ml of
glycerol. Heat the medium to dissolve completely, autoclave at 121°C for 15 minutes. Prepare
1000 ml of a uniform suspension of fresh eggs under aseptic conditions. Swirl gently to avoid
introducing air into the suspension. Aseptically mix the 1000 ml of egg suspension with 600 ml of
the sterile Lowenstein-Jensen Medium base cooled to 50 – 60°C, avoiding air bubbles. Dispense
the finished medium into sterile screw-cap test tubes. Place the tubes in a slanted position and
heat at 85°C for 45 minutes to coagulate the medium.
