For maintaining and propagating Escherichia coli in molecular microbiology procedures.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Light yellow to amber colored opalescent
pH
7.0±0.2 at 25˚C (2 5.00 g/ l)
Contents
Ingredients
Gram/Litre
Tryptone
10.000
Yeast Extract
5.000
Sodium Chloride
10.000
pH at 25˚C
7.0 ±0.2
Directions
Dissolve 25.00 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C
and inoculate test sample aseptically.
