A selective medium used for the isolation of Gram-negative bacteria from urine & faeces.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light beige with green ting colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Green blue colored, clear, opalescent solution
pH
8.6±0.2 at 25˚C (33.10 g/ l)
Solidification
40˚C
Color
Green blue colored, clear opalescent gel
Contents
Ingredients
Gram/Litre
Proteose peptone
5.000
Meat extract
3.000
Lactose
10.000
Bromothymol blue
0.170
Agar
15.000
pH at 25˚C
8.6 ±0.2
Directions
Dissolve 33.1 grams in 1000 ml distilled water. Heat to boiling to dissolve the
medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C), cool to 45-50°C. Mix well
and distribute aseptically in petri plates and allow to solidify. Ensure complete solidification and
inoculate test sample aseptically.
