For differentiation of Escherichia coli and Enterobacter aerogenes based on citrate
utilization
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
White colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Colorless clear solution
pH
6.7±0.2 at 25˚C (5.70 g/ l)
Contents
Ingredients
Gram/Litre
Sodium Ammonium Phosphate
1.500
Monobasic Potassium Phosphate
1.000
Magnesium Sulfate
0.200
Sodium Citrate
3.000
pH at 25˚C
6.7±0.2
Directions
Dissolve 5.7 grams in 1000 ml distilled water, boil to dissolve the medium
completely and distribute aseptically in test tubes. Sterilize by autoclaving at 15 lbs pressure (121
°C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.
