For detection and cultivation of nitrogen fixing bacteria.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Off white colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Milky white colored opalescent solution with slight precipitate
pH
7.20 to 7.80 at 25˚C (24.10 g/ l)
Contents
Ingredients
Gram/Litre
Sucrose
20.000
Dipotassium Phosphate
1.000
Magnesium Sulphate
0.500
Sodium Chloride
0.500
Ferrous Sulphate
0.100
Sodium Molybdate
0.005
Calcium Carbonate
2.000
pH at 25˚C
7.20to 7.80
Directions
Dissolve 24.10 grams in 1000 ml distilled water. Boil to dissolve the medium
completely. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and
inoculate test sample aseptically.
