For the detection and cultivation of nitrogen fixing bacteria.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Off white colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Milky white colored opalescent with slight precipitate gel
pH
7.20 to 7.80 at 25˚C (39.10 g/ l)
Solidification
40˚C
Color
Off white colored opalescent gel
Contents
Ingredients
Gram/Litre
Sucrose
20.000
Dipotassium Phosphate
1.000
Magnesium Sulphate
0.500
Sodium Chloride
0.500
Ferrous Sulphate
0.100
Sodium Molybdate
0.005
Calcium Carbonate
2.000
Agar
15.000
pH at 25˚C
7.20 to 7.80
Directions
Dissolve 39.10 grams in 1000 ml distilled water. Boil to dissolve the medium
completely. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and
distribute in desired. Allow to solidify and inoculate test sample aseptically.