For detection and enumeration of coliform organisms by a fluorogenic method.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Pink-beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Reddish-purple, slightly opalescent solution
pH
7.4 ±0.2 at 25℃ (41.63 g/ l)
Solidification
40˚C
Color
Reddish-purple, slightly opalescent gel
Contents
Ingredients
Gram/Litre
Peptone
7.000
Yeast Extract
3.000
Bile Salts
1.500
Sodium Chloride
5.000
Lactose
10.000
Neutral Red
0.030
Crystal Violet
0.002
4-Methylumbelliferyl β-D-glucuronide (MUG)
0.100
Agar
15.000
pH at 25˚C
7.4 ±0.2
Directions
Dissolve 41.63 grams in 1000 ml distilled water. Heat to boil for 1
minutes to dissolve ingredients completely, Do Not Autoclave. In aseptic condition
immediately cool it to 42-45°C and distribute in sterile petri plates and allow solidify.
Ensure complete solidification and inoculate test sample.
