For improved enrichment and isolation of Salmonellae.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Green colored opalescent solution
Complete medium with added iodine solution and Brilliant green solution (Color)
Light green opalescent with white precipitate, on standing the precipitate settles down.
Contents
Ingredients
Gram/Litre
Casein peptone
7.000
Soya peptone
2.300
Sodium chloride
2.300
Calcium carbonate
25.000
Sodium thiosulphate
40.700
Ox bile
4.750
Directions
Dissolve 82.00 g in 970 ml distilled water and heat just too boiling. Cool below to 45
50 ˚C, and add 20 ml iodine solution, and 10 ml of 0.095% brilliant green solution. Mix well and
distribute aseptically in sterile test tubes in such a manner to distribute equal amount of calcium
carbonate in all tubes. Inoculate test sample aseptically.
Preparation of Iodine solution: dissolve 5 g of potassium iodide and 4 g of iodine in 20 ml distilled
water.
