For testing performing Antifungal Disk Diffusion Susceptibility of yeasts
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Light yellow with slight blue tinge.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Light yellow with slight greenish tinge solution
pH
7.3 ±0.1 at 25 ˚C (58.00 g/ l)
Solidification
40˚C
Color
Light yellow with slight greenish tinge opalescent gel
Contents
Ingredients
Gram/Litre
Meat Infusion Form
300.000
Casamino Acids
17.500
Starch
1.500
Glucose
20.000
Methylene blue
0.0005
Agar
17.000
pH at 25˚C
7.3 ±0.1
Directions
Dissolve 58.00 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs. pressure (121 °C) for 15 min, cool it to 42-45 °C
and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample
aseptically.
