To determine the mutagenic potential of chemicals and drugs.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Off white to beige colored.
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Light to medium amber colored, slightly opalescent gel
pH
7.00 ±0.2 at 25 ˚C (48.7 g/ l)
Solidification
40˚C
Contents
Ingredients
Gram/Litre
Glucose
20.000
Dipotassium hydrogen phosphate
10.000
Sodium hydrogen phosphate
3.500
Citric acid
2.000
Magnesium sulphate
0.200
Agar
13.000
pH at 25˚C
7.0 ± 0.2
Directions
Dissolve 48.7 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C
and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample
aseptically.
