For the enumeration of Pseudomonas aeruginosa in waters.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Part A: Free flowing, homogeneous powder Part B: Free flowing, homogeneous powder
Solubility
Soluble in Distilled / de-ionized water at 50˚ C
Color
Part A: Off white Part B: Light beige
pH
Light amber colored opalescent
Solidification
40˚C
Color
Light amber colored opalescent gel
Contents
Ingredients
Gram/Litre
Part A
Skim Milk Powder
100.00
Part B
Peptone
5.00
Meat Extract
1.50
Yeast Extract
1.50
Sodium Chloride
5.00
Agar
15.00
pH at 25˚C
7.4 ±0.2
Directions
Part A: Dissolve 100.00 grams in 500 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs. pressure (121°C) for 5 min, cool it to room
temperature.
Part B: Dissolve 28.00 grams in 500 ml distilled water. Boil to dissolve the medium completely
and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 55 °C.
Mix Part A and Part B in sterile flask and distribute aseptically in sterile petri plates and allow
to solidify. Ensure complete solidification and inoculate test organisms aseptically.
