For the isolation and enumeration of Clostridium perfringens from water samples using
membrane filtration technique.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Light greenish yellow colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Purple colored clear to slightly opalescent
pH
7.6 ±0.2 at 25 ˚C (35.60 g / 485 ml)
Solidification
40˚C
Color
Purple colored clear to slightly opalescent gel
Contents
Ingredients
Gram/Litre
Tryptose
30.00
Yeast Extract
20.00
Sucrose
5.00
L-Cysteine
1.00
Magnesium Sulphate
0.10
Bromocresol Purple
0.04
Ferric Chloride
0.09
Indoxyl-Β-D-Glucoside
0.06
Agar
15.00
pH at 25˚C
7.6 ±0.2
Directions
Dissolve 35.60 grams in 485 ml distilled water, boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C,
aseptically add sterile 5.0 ml of D-cycloserine (200mg) solution and Polymyxin B sulphate
(12.500mg) and 10 ml Phenolphthalein diphosphate (0.05g) solution. Mix well and distribute
aseptically in petri plates. Ensure complete solidification and inoculate test sample aseptically.
