A protective and isotonic diluent used for maximal recovery of microorganisms.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Off white to light Beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Pale yellow color clear solution
pH
7.0 ±0.2 at 25℃ (9.50 g/ l)
Contents
Ingredients
Gram/Litre
Peptone
1.000
Sodium Chloride
8.500
pH at 25˚C
7.0 ±0.2
Directions
Dissolve 9.50 grams in 1000 ml distilled water, boil to dissolve the medium
completely. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool. Dissolve 10 gm of
test sample in 90 ml of Maximum Recovery Diluent solution blend it at 15,000 to 20,000
revolutions per minute. Further a tenfold dilution may be prepared using 1.00 ml of it in 9.00 ml
of sterile diluent within 15 minutes and mixed well. This is considered as 10-1 dilution. Sequential
dilutions can be prepared using same diluent and counts obtained by spread plate or pour plate
technique.
