For the enrichment of Salmonellae from faeces, food and other materials.
Specifications
PHYSICAL PARAMETERS
Description
Part A and B fine, free flowing, powder.
Color
Part A: Cream to pale green Part B: White to cream
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Green colored Opalescent to slightly hazy solution of complete medium
pH
7.0 ±0.2 at 25˚C (Part A 2.37% w/v & Part B 0.4% w/v)
Contents
Ingredients
Gram/Litre
Part A
Peptone
5.000
Yeast extract
5.000
Sodium taurocholate
1.000
Brilliant green
0.005
Potassium dihydrogen phosphate
3.400
Dipotassium hydrogen phosphate
4.350
Mannitol
5.000
Part B
Sodium selenite
4.000
pH at 25°C
7.0±0.2
Directions
Suspend 4.0grams of Part B in 1000 ml purified/distilled water. Add 23.75grams of
Part A. Mix well. If desired add 0.5g/L sodium sulpha pyridine, warm to dissolve the medium
completely. Dispense as desired and sterilize in a boiling water bath or free flowing steam for 10
minutes. DO NOT AUTOCLAVE. Excessive heating is detrimental. Discard the prepared
medium if large amount of selenite is reduced (indicated by red precipitate at the bottom of
tube/bottle).
