For the isolation and cultivation of Staphylococci in accordance with microbial limit
testing by harmonized methodology of USP/EP/BP/JP/IP.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Light pink beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Orange to red colored opalescent solution
pH
7.40 ±0.2 at 25℃ (111.02 g/ l)
Solidification
40˚C
Color
Orange to red colored slightly opalescent gel
Contents
Ingredients
Gram/Litre
Pancreatic Digest of Casein
5.000
Peptic Digest of Animal Tissue
5.000
Meat Extract
1.000
D-Mannitol
10.000
Sodium Chloride
75.000
Phenol Red
0.025
Agar
15.000
pH at 25˚C
7.4 ±0.2
Directions
Dissolve 111.02 grams in 1000 ml distilled water. Boil to dissolve the medium
completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C, and
distribute in sterile petri plates and allow to solidify. Ensure complete solidification and inoculate
test sample aseptically.
