For the differentiation of members of Enterobacteriaceae on the basis of malonate
utilization.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Beige colored with green ting.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Blue green colored clear solution
pH
6.70 ±0.2 at 25℃ (9.28 g/ l)
Contents
Ingredients
Gram/Litre
Yeast extract
1.000
Ammonium sulphate
2.000
Dipotassium phosphate
0.600
Potassium dihydrogen phosphate
0.400
Sodium chloride
2.000
Sodium malonate
3.000
Dextrose
0.250
Bromothymol blue
0.025
pH at 25˚C
6.7 ±0.2
Directions
Dissolve 9.28 grams in 1000 ml distilled water. Heat, if necessary, to dissolve the
medium completely. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, Cool to 20
25°C and aseptically inoculate test sample.
