For isolation and enumeration of coliforms in water, milk and dairy products.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder.
Color
Pinkish beige colored powder.
Solubility
Soluble in Distilled/de-ionized water at 50°C
Color
Orange red colored, slightly opalescent gel
pH
7.1 ± 0.2 at 25 ˚C (42.53 g/ l)
Solidification
40 ˚C
Color
Orange red colored opalescent gel
Contents
Ingredients
Gram/Litre
Peptone
10.000
Lactose
10.000
Sodium chloride
5.000
Sodium citrate
2.000
Sodium Deoxycholate
0.500
Neutral Red
0.030
Agar
15.000
pH at 25˚C
7.1 ± 0.2
Directions
Dissolve 42.53 grams in 1000 ml distilled water. Boil to dissolve the medium completely. DO NOT STERILIZE BY AUTOCLAVING, cool it to 42-45 °C and distribute aseptically in to the petri plates. Ensure complete solidification and inoculate test sample aseptically.
Note: It the medium is store it should be sterilized by autoclave at 121℃ (15 lbs) for 15 minutes.
