For selective and confirmatory detection of Enterococci as an indicator of faecal pollution in water.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Light beige colored powder.
Solubility
Soluble in Distilled/de-ionized water at 50°C
Color
Light amber colored opalescent
pH
7.0±0.2 at 25˚C (35.80 g/ l)
Contents
Ingredients
Gram/Litre
Tryptose
20.000
Dextrose
5.000
Dipotassium Phosphate
2.700
Monopotassium Phosphate
2.700
Sodium Chloride
5.000
Sodium Azide
0.400
Ethyl Violet
0.0008
pH at 25˚C
7.0 ±0.2
Directions
Dissolve 35.80 grams in 1000 ml distilled water. Boil to dissolve the medium completely and distribute aseptically. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.
