For the detection of lactose fermenting enteric bacteria.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Beige to greenish yellow colored powder.
Solubility
Soluble in Distilled/de-ionized water at 50°C
Color
Greenish color to slightly to medium opalescent gel
pH
7.1 ± 0.2 at 25 ˚C (51.5.00 g/ l)
Solidification
40 ˚C
Contents
Ingredients
Gram/Litre
Peptone
20.000
Lactose
10.000
Bile salt
1.500
Sodium chloride
5.000
Bromothymol Blue
0.030
Agar
15.000
pH at 25˚C
7.1 ± 0.2
Directions
Dissolve 51.5 grams in 1000 ml distilled water, boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample aseptically.
