For selective isolation and differentiation of Escherichia coli and coliform bacteria in
pharmaceutical testing according to harmonized methods.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Pinkish beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Orange red, slightly opalescent solution
pH
7.1 ±0.2 at 25 ˚C (50.00 g/ l)
Solidification
40˚C
Color
Pinkish red, slightly opalescent gel
Contents
Ingredients
Gram/Litre
Pancreatic Digest of Gelatin
17.000
Peptone (Meat and Casein)
3.000
Lactose Monohydrate
10.000
Sodium Chloride
5.000
Bile Salts
1.500
Neutral Red
0.030
Crystal Violet
0.001
Agar
13.500
pH at 25˚C
7.1 ±0.2
Directions
Dissolve 51.50 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C
and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample
aseptically.
