For the detection and isolation of Pseudomonas aeruginosa by membrane filter technique.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Light pink beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Orange red colored opalescent
pH
7.1 ±0.2 at 25˚C (40.00 g/ l)
Solidification
40˚C
Color
Orange red colored opalescent gel
Contents
Ingredients
Gram/Litre
L-Lysine
5.000
Yeast Extract
2.000
Xylose
2.500
Sucrose
1.250
Lactose
1.250
Sodium Chloride
5.000
Ferric Ammonium Citrate
0.800
Sodium Thiosulphate
6.800
Phenol Red
0.080
Agar
15.000
pH at 25˚C
7.1 ±0.2
Directions
Dissolve 40.00 grams in 1000 ml distilled water, boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 min, cool it to 42-45 °C
and add 5.0 ml of sterile antibiotic solution (Sulphapyridine (176 mg) Kanamycin (8.50 mg),
Nalidixic acid (37 mg) and Amphotericin B (5.0 mg)). Mix well and distribute aseptically in
sterile petri plates. Ensure complete solidification and inoculate test sample aseptically.
