For selective isolation of pathogenic Clostridia from mixed flora.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Light Beige colored powder.
Solubility
Soluble in Distilled/de-ionized water at 50°C
Color
Light amber colored opalescent
pH
7.0 ±0.2 at 25 ˚C (47.00 g/ l)
Solidification
40 ˚C
Color
Light amber colored opalescent gel
Contents
Ingredients
Gram/Litre
Tryptone
17.000
Soya Peptone
3.000
Dextrose
6.000
Sodium Chloride
2.500
Sodium Thioglycolate
1.800
L-Cystine
0.250
Sodium Formaldehyde Sulphoxylate
1.000
Neomycin Sulphate
0.150
Sodium Azide
0.200
Agar
15.000
pH at 25˚C
7.0 ±0.2
Directions
Dissolve 47.00 grams in 1000 ml distilled water, boil to dissolve the medium completely and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C and distribute aseptically in petri plates. Ensure complete solidification and inoculate test sample aseptically.
