For the selective isolation and identification of Pseudomonas aeruginosa in pharmaceutical
testing according to harmonized methods.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Light amber colored opalescent solution with a slight precipitate
pH
7.2 ±0.2 at 25 ˚C (45.50 g/ l)
Solidification
40˚C
Color
Light amber colored opalescent gel
Contents
Ingredients
Gram/Litre
Pancreatic Digest of Gelatin
20.000
Magnesium Chloride
1.400
Dipotassium Sulfate
10.000
Cetrimide
0.300
Agar
13.600
pH at 25˚C
7.2 ±0.2
Directions
Dissolve 45.50 grams in 1000 ml distilled water containing 1% glycerol. If
necessary, boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure
(121°C) for 15 min, cool it to 42-45°C and distribute aseptically in sterile petri plates and allow to
solidify. Ensure complete solidification and inoculate test sample aseptically.
