For cultivation of Brucella species and for isolation and subculture of anaerobes by adding
blood.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Cream to light brown colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Light amber colored opalescent
pH
7.4 ±0.2 at 25 ˚C (50.00 g/ l)
Solidification
40˚C
Color
Light amber colored opalescent gel
Contents
Ingredients
Gram/Litre
Peptone
10.00
Meat extract
5.00
Dextrose
10.00
Sodium chloride
5.00
Agar
15.00
pH at 25˚C
7.5±0.2
Directions
Dissolve 45.0 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45°C
and aseptically add 50 ml of sterile bovine serum and 10 ml selective supplement containing
(Bacitracin – 25000 IU, Polymyxin B 5000 IU, Vancomycin- 0.02 g, nalidixic acid – 0.005 g and
cyloheximide 0.1 g per liter), mix well and distribute in petri plates and allow to solidify. Ensure
complete solidification and inoculate test sample aseptically.
