For selective isolation & cultivation of Brucella or Campylobacter species from clinical as
well as nonclinical specimens.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Cream to light brown colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Beige colored opalescent
pH
7.0 ±0.2 at 25℃ (43.00 g/ l)
Solidification
40˚C
Color
Light yellow to creamy colored opalescent gel
Contents
Ingredients
Gram/Litre
Tryptone
10.00
Peptone
10.00
Yeast extract
2.00
Dextrose
1.00
Sodium chloride
5.00
Sodium bisulphite
0.10
Agar
15.00
pH at 25˚C
7.0 ±0.2
Directions
Dissolve 43.00 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 min, cool it to 42-45 °C
and distribute in petri plates and allow to solidify. Ensure complete solidification and inoculate
test sample aseptically.
Elective: To prepare Brucella Blood Agar, aseptically add 5-10% sterile defibrinated blood at 45
50°C. Mix well.
