For isolation and cultivation of various fastidious pathogenic microorganisms after
addition of blood
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Dark amber, slightly opalescent
pH
7.4 ±0.2 at 25 ˚C (40.00 g/ l)
Solidification
40˚C
Color
Without blood: dark amber, slightly opalescent gel. With 5% blood: cherry red, opaque gel.
Contents
Ingredients
Gram/Litre
Peptone
10.000
Tryptone
10.000
Sodium Chloride
5.000
Agar
15.000
pH at 25˚C
7.4 ±0.2
Directions
Dissolve 40.00 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs. pressure (121 °C) for 15 min, cool it to 42-45 °C,
and aseptically add 5% v/v sterile defibrinated blood. Mix well and pour into sterile petri plates.
Ensure complete solidification and inoculate test sample aseptically.
