For isolation and enumeration of coagulase positive Staphylococci from food and other
substance.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Light yellowish colored opalescent
pH
7.0 ± 0.2 at 25 ˚C (63.00 g/ l)
Solidification
40˚C
Color
Light yellowish colored opalescent gel
Contents
Ingredients
Gram/Litre
Tryptone
10.000
Meat Extract
5.000
Yeast Extract
1.000
Glycine
12.000
Sodium Pyruvate
10.000
Lithium Chloride
5.000
Agar
20.000
pH at 25˚C
6.9 ±0.2
Directions
Dissolve 63.00 grams in 950 ml distilled water, boil to dissolve the medium completely
and sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C. After
cooling to 45- 50°C, add 50 mL of Egg Yolk Tellurite Supplement and 3 ml sterile 3.5%
Potassium Tellurite solution or 50 ml Egg Yolk Tellurite Emulsion. Mix thoroughly before
dispensing.
