For the isolation and enumeration of Bacillus cereus.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Greenish Beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Green color solution
pH
7.2 ±0.2 at 25 ˚C (41.00 g/ l)
Solidification
40˚C
Color
Green color, clear to slightly opalescent gel, after addition of egg yolk emulsion: yellowish green
Contents
Ingredients
Gram/Litre
Peptone
1.000
Mannitol
10.000
Sodium Chloride
2.000
Magnesium sulphate
0.100
Disodium phosphate
2.500
Monopotassium phosphate
0.250
Sodium pyruvate
10.000
Bromot hymol blue
0.120
Agar
15.000
pH at 25˚C
7.2 ±0.2
Directions
Dissolve 41.00 grams in 950 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs. pressure (121 °C) for 15 min, cool it to 42-45 °C,
and aseptically add Polymyxin B (so as to get 100 units/ml) and 50 ml sterile egg yolk emulsion.
Mix well and pour into sterile petri plates. Avoiding air bubbles while pouring. Ensure complete
solidification and inoculate test sample aseptically.
