For isolation, cultivation and identification of Glucose positive Azotobacter species from
soil.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Light beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Clear slightly opalescent solution
pH
7.6 ±0.2 at 25℃ (10.40 g/ l)
Contents
Ingredients
Gram/Litre
Dipotassium Hydrogen Phosphate
0.050
Potassium Hydrogen Phosphate
0.150
Magnesium Sulphate
0.200
Calcium Chloride
0.020
Sodium Ammonium Molybdate
0.002
Ferric Chloride
0.00009
Glucose
10.000
pH at 25˚C
7.6 ± 0.2
Directions
Dissolve 10.40 grams in 1000 ml distilled water, boil to dissolve the medium
completely. Sterilize by autoclaving at 15 lbs. pressure (121 °C) for 15 min, cool it to 42-45 °C
and inoculate test sample aseptically.
