Part A: Cream to yellow homogeneous free flowing powder Part B: White to cream pellets.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Light yellow to pale green colored solution
pH
6.8 ±0.2 at 25℃ (8.10g part A and 4g part B)
Contents
Ingredients
Gram/Litre
Part A
Malic acid
5.000
Dipotassium hydrogen phosphate
0.500
Ferrous Sulphate
0.500
Manganese Sulphate
0.010
Magnesium Sulphate
0.200
Sodium Chloride
0.100
Bromothymol blue
0.002
Sodium molybdate
0.002
Calcium chloride
0.020
Agar
1.750
Part B
Potassium hydroxide
4.000
pH at 25˚C
6.8 ±0.2
Directions
Part B: Dissolve 4 grams of part B in 50 ml of sterile distilled water. Part A:
Dissolve 8.10 grams in 950 ml distilled water, boil to dissolve the medium completely. Sterilize by
autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C. Mix 950 ml of part A with
50 ml of part B in 1 liter sterile container, mix well and dispense desired.
