Azide Dextrose Broth

ARTICLE NO. OLFC-285
HSN NO. 38210000


Pack Size

Size
100 GM
500 GM

Application

For detection of faecal Streptococci in water, sewage, food and other materials.


Specifications

PHYSICAL PARAMETERS
Description A fine, free flowing, hygroscopic powder
Color Beige colored powder.
Solubility Soluble in Distilled / de-ionized water at 50˚C
Color Light amber colored clear solution
pH 7.2 ±0.2 at 25 ˚C (34.7.00 g/ l)

Contents

Ingredients Gram/Litre
Tryptose15.00
Meat extract4.50
Dextrose7.50
Sodium chloride7.50
Sodium azide0.20
pH at 25˚C7.2 ±0.2


Directions

Dissolve 34.70 grams in 1000 ml distilled water. Boil to dissolve the medium completely and distribute aseptically. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically. For double strength media preparation dissolve twice amount (69.40 g/l) of dehydrated media in 1000 ml distilled water.