For detection of aflatoxin producing Aspergillus species from food samples.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Beige colored powder.
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Light amber, slightly opalescent
pH
6.3 ±0.2 at 25 ˚C (45.50 g/ l)
Solidification
40˚C
Color
Light amber color opalescent gel
Contents
Ingredients
Gram/Litre
Peptone
10.000
Yeast Extract
20.000
Ferric ammonium citrate
0.500
Dichloran
0.002
Agar
15.000
pH at 25˚C
6.3 ±0.2
Directions
Dissolve 45.50 grams in 1000 ml distilled water. Boil to dissolve the medium
completely and sterilize by autoclaving at 15 lbs. pressure (121 °C) for 15 min, cool it to 42-45 °C,
aseptically add Chloramphenicol Selective Supplement (100 mg). Mix well and pour into sterile
petri plates. Avoiding air bubbles while pouring. Ensure complete solidification and inoculate
test sample aseptically.
