Ashby’s Sucrose Broth

ARTICLE NO. OLFC-270
HSN NO. 38210000


Pack Size

Size
100 GM
500 GM

Application

For growth and maintenance of Azotobacter species.


Specifications

PHYSICAL PARAMETERS
Description A fine, free flowing, hygroscopic powder
Color Off white colored powder.
Solubility Soluble in Distilled / de-ionized water at 50˚C
Color Milky white colored solution with stealing at bottom on standing
pH 7.4 ±0.2 at 25℃ (25.70 g/ l)

Contents

Ingredients Gram/Litre
Sucrose20.00
Dipotassium Phosphate0.20
Magnesium Sulphate0.20
Sodium Chloride0.20
Potassium Sulphate0.10
Calcium Carbonate5.00
pH at 25˚C7.4 ±0.2


Directions

Dissolve 25.70 grams in 1000 ml distilled water. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.