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Ashby’s Mannitol Broth

ARTICLE NO. OLFC-260
HSN NO. 38210000

MSDS

TDS(IFU)


Pack Size

Size
100 GM
500 GM

Application

For isolation of Azotobacter species from soil.


Specifications

PHYSICAL PARAMETERS
Description A fine, free flowing, hygroscopic powder
Color Off white colored powder.
Solubility Soluble in Distilled / de-ionized water at 50˚C
Color Milky white colored solution with stealing at bottom on standing.
pH 7.4 ±0.2 at 25℃ (25.70 g/ l)

Contents

Ingredients Gram/Litre
Mannitol20.00
Dipotassium Phosphate0.20
Magnesium Sulphate0.20
Sodium Chloride0.20
Potassium Sulphate0.10
Calcium Carbonate5.00
pH at 25˚C7.4 ±0.2


Directions

Dissolve 25.70 grams in 1000 ml distilled water. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 min, cool it to 42-45 °C and inoculate test sample aseptically.