For isolation of Azotobacter species from soil that can use mannitol and atmospheric
nitrogen as source of carbon and nitrogen respectively.
Specifications
PHYSICAL PARAMETERS
Description
A fine, free flowing, hygroscopic powder
Color
Off white colored powder
Solubility
Soluble in Distilled / de-ionized water at 50˚C
Color
Milky white colored solution with stealing at bottom on standing
pH
7.4 ±0.2 at 25℃ (40.70 g/ l)
Solidification
40˚C
Color
Milky white colored opalescent gel with slight precipitate
Contents
Ingredients
Gram/Litre
Mannitol
20.00
Dipotassium Phosphate
0.20
Magnesium Sulphate
0.20
Sodium Chloride
0.20
Potassium Sulphate
0.10
Calcium Carbonate
5.00
Agar
15.00
pH at 25˚C
7.4 ±0.2
Directions
Dissolve 40.70 grams in 1000 ml distilled water. Sterilize by autoclaving at 15 lbs
pressure (121 °C) for 15 min, cool it to 42-45 °C and distribute in desire. Ensure complete
solidification and inoculate test sample aseptically.
